First GMO Mosquitoes to Be Released In the Florida Keys
This spring, the biotechnology company Oxitec plans to release genetically modified (GM) mosquitoes in the Florida Keys. Oxitec says its technology will combat dengue fever, a potentially life-threatening disease, and other mosquito-borne viruses — such as Zika — mainly transmitted by the Aedes aegypti mosquito.
While there have been more than 7,300 dengue cases reported in the United States between 2010 and 2020, a majority are contracted in Asia and the Caribbean, according to the U.S. Centers for Disease Control and Prevention. In Florida, however, there were 41 travel-related cases in 2020, compared with 71 cases that were transmitted locally.
Native mosquitoes in Florida are increasingly resistant to the most common form of control — insecticide — and scientists say they need new and better techniques to control the insects and the diseases they carry. “There aren’t any other tools that we have. Mosquito nets don’t work. Vaccines are under development but need to be fully efficacious,” says Michael Bonsall, a mathematical biologist at the University of Oxford, who is not affiliated with Oxitec but has collaborated with the company in the past, and who worked with the World Health Organization to produce a GM mosquito-testing framework.
Bonsall and other scientists think a combination of approaches is essential to reducing the burden of diseases — and that, maybe, newer ideas like GM mosquitoes should be added to the mix. Oxitec’s mosquitoes, for instance, are genetically altered to pass what the company calls “self-limiting” genes to their offspring; when released GM males breed with wild female mosquitoes, the resulting generation does not survive into adulthood, reducing the overall population.
But Oxitec has been proposing to experimentally release GM mosquitos in the Keys since 2011, and the plan has long been met with suspicion among locals and debate among scientists. Some locals say they fear being guinea pigs. Critics say they are concerned about the possible effects GM mosquitoes could have on human health and the environment. In 2012, the Key West City Commission objected to Oxitec’s plan; in a non-binding referendum four years later, residents of Key Haven — where the mosquitoes would have been released — rejected it, while residents in the surrounding county voted in support of the release. With the decision left up to the Florida Keys Mosquito Control District, officials approved the trial to be conducted elsewhere in the Keys.
According to Oxitec, the release was delayed due to a transfer of jurisdiction over the project from the U.S. Food and Drug Administration to the Environmental Protection Agency.
The company reapplied for approval to release a new version of the mosquitoes, called OX5034, in the Keys. In May, the EPA granted a two-year experimental use permit, which the agency can cancel at any time. State and local sign-off soon followed — finally giving the project the greenlight.
Oxitec has been proposing to experimentally release GM mosquitos in the Keys since 2011, and the plan has long been met with suspicion.
Oxitec’s OX5034 mosquitoes are the first GM mosquitoes approved for release in the U.S. The company has already conducted a trial with the OX5034 mosquitoes in Brazil and released more than a billion of a previous version, called OX513A, there and in other locations over the years — including the Cayman Islands. The company says it is confident in the effectiveness and safety of the technology.
But some scientists want to hit pause on Oxitec’s Florida trial, to find what they say is a fairer process in deciding to release the mosquitoes. Others want to see clearer proof that this technology is even necessary, claiming that the company has only released its most positive data with the public and has kept other key data, including whether the mosquitoes curb disease transmission, private. And if the release actually launches as planned, some Keys residents say they aim to interfere.
Critics also say that Oxitec failed to engage with local communities in Florida and get their consent to release the mosquitoes. “What’s the most upsetting is that the very people that are going to be most impacted, both by the benefits or the risks of such a decision, have like the smallest voice in how these choices are made. I think that’s a really big issue,” says Natalie Kofler, a molecular biologist and bioethicist who founded Editing Nature, a platform that advocates “for inclusive decision-making processes to steer” the use of genetic technology. “If Oxitec doesn’t do this right,” she adds, “we could have huge impact on delaying the use of other beneficial technologies like that in the future.”
Oxitec’s OX5034 mosquitoes are programmed to combat the transmission of mosquito-borne illnesses by suppressing local Aedes aegypti populations. Oxitec — which is U.S.-owned and based in the United Kingdom — describes their mosquitoes as “friendly” because they will only release males, which, unlike females, do not bite humans or transmit disease.
At Oxitec’s laboratory in the U.K., the company genetically engineers the mosquitoes, giving the insects the “self-limiting” gene that makes the females dependent on the antibiotic tetracycline. Without the drug, they will die. Eggs from these genetically-altered mosquitoes — which will hatch both male and female insects — will be shipped to the Keys. Mosquitoes require water to mature from an egg to an adult; when Oxitec’s team adds water to the boxes the mosquitoes will be deployed in, both GM males and GM females will hatch. With no tetracycline present in the box, the GM females are expected to die in early larval stages.
The male mosquitoes will survive and carry the gene. When they leave the boxes, the insects will, hypothetically, fly away to mate with wild females to pass the gene to the next wild generation, according to Nathan Rose, head of regulatory affairs at Oxitec. Kevin Gorman, the company’s chief development officer, says the local female mosquito population will be increasingly reduced — which will also reduce the number of wild male mosquitoes in the treatment areas.
Gorman emphasized to Undark that the EPA and other regulators found no risk in using tetracycline in breeding their genetically-altered mosquitoes. But some scientists think the presence of this antibiotic in the environment does pose a risk. According to Jennifer Kuzma, co-founder and co-director of the Genetic Engineering and Society Center at North Carolina State University, tetracycline is commonly found in sewage, and it is also used in Florida to prevent bacterial diseases in agriculture — particularly in citrus groves. The presence of the antibiotic may mean that it will remain in the environment, especially in water where the mosquitoes breed, which could allow Oxitec’s female mosquitoes to survive. While the company does not plan to release the mosquitos near areas where the antibiotic is used, Kuzma says the EPA’s risk assessment did not include testing of any standing water for tetracycline — something, she adds, “would have been easy enough to do for good due diligence.”
“What’s the most upsetting is that the very people that are going to be most impacted, both by the benefits or the risks of such a decision, have like the smallest voice in how these choices are made. I think that’s a really big issue,” says Kofler
Skeptics of Oxitec’s GM mosquitoes include local residents, physicians, scientists, and environmental activists. Many of these opponents say they aren’t anti-GMO, but disagree with how the approval process has been handled. One group has even kept a running list of what it sees as Oxitec’s wrongdoings since it first began experimental releases. The list includes Oxitec’s lack of disease monitoring in the countries where it has released mosquitoes; the unknown price of its technology; and complaints that the company has overstated the success of some of it its trials.
“I cannot trust this company. I cannot trust this technology,” says Mara Daly, a resident of Key Largo who says she’s been following Oxitec’s plans for nine years.
“This is not a traditional pesticide,” she adds. “ This is not a chemical that you can trace. This is something completely different, new emerging technology and we need better regulation.”
Phil Goodman, chairman of the Florida Keys Mosquito Control District (FKMCD), an independently-elected commission carrying out mosquito control within Monroe County, says that many of those who discredit Oxitec’s evidence do not understand the technology. “They’re fearmongering,” he says.
“They have very little credibility here in the Florida Keys as far as I’m concerned,” he adds.
But people like Daly and Barry Wray, executive director of the Florida Keys Environmental Coalition, disagree. “We want to know it’s safe,” says Wray, who notes that his group more generally supports GM technology. “We don’t have another Florida Keys ecosystem. We don’t have another Florida Keys community. We have this one.”
Daly, Wray, and others point to what they perceive as the FKMCD’s disrespect for public opinion. They argue that the community wasn’t given a chance to consent before the EPA approval. There was a 30-day public forum in September 2019 about Oxitec’s technology application, with 31,174 comments opposing release and 56 in support. A statement emailed to Undark by Melissa Sullivan, an EPA spokesperson, noted that the agency considered these comments during the review, but critics think it happened too quickly to be of real use.
In June, Kofler and Kuzma wrote an opinion piece in The Boston Globe about the EPA approval, critiquing the agency’s regulatory system and calling for a better process for evaluating new biotechnologies. The researchers expressed concern that “the EPA did not convene an independent, external scientific advisory panel to review” Oxitec’s claims about its mosquito strategy and that the agency only publicly released its risk assessment after approving the technology. The “American public,” Kofler and Kuzma wrote, “needs to be assured that these decisions are made free of conflicts of interest.” The statement from the EPA’s Sullivan noted that the agency “conducted an extensive risk assessment based on the best available science.”
Some critics also wanted there to be more public engagement. Kofler and Kuzma say they offered to provide their expertise, along with other outside experts, to the mosquito control district to allow more discussion about the GM mosquitoes with the Keys community. But Kofler says the district wasn’t responsive. Oxitec itself launched webinars about their new product, but not until after the EPA approval. “Here we are, like in the final hour, having these conversations that needed to be happening a year ago,” says Kofler.
Without public trust and enthusiasm, it doesn’t matter whether Oxitec’s mosquito technique works, says Guy Reeves, a genetic researcher at the Max Planck Institute for Evolutionary Biology in Germany, who stresses that he doesn’t think the company’s approach is unsafe. “If the population in Florida Keys becomes so sensitized to this issue — that they can no longer cooperate with each other — that’s good for the mosquitoes, not good for the people,” he adds.
Based on their first generation mosquito OX513A, Oxitec says it has shown that the approach reduces a targeted mosquito population in trials in both Brazil and the Cayman Islands. But there’s no evidence that this new OX5034 mosquito release will actually be worth it for mosquito suppression, says Reeves. Oxitec also hasn’t explained how their new mosquito will directly curb human diseases, such as dengue. Reducing disease transmission and burden should be measures of efficacy for this technology, says Kofler.
According to Gorman, independent disease suppression data has only been collected by municipalities in Brazil because that’s where most of the company’s trials have been released in larger scales. These municipalities have shown that Oxitec mosquitoes have reduced dengue cases in areas of release, Gorman says. In order for Oxitec to collect additional data, he adds, the company needs to release and test large areas over sustained periods of time. Gorman maintains that the company is not required to report formal health impact studies.
Reeves adds that Oxitec also hasn’t explained what resources are needed to sustain this product, how long it could take to be effective, or the cost. When asked about the cost of the Florida Keys project, Oxitec responded to Undark by email: “Oxitec is a pre-commercial, pre-profit company. We will not profit from this pilot project in Florida. We are paying for it ourselves.”
Oxitec has released more than a billion of their OX513A mosquitoes over the past 10 years. According to independent scientists, some of those experiments did not go well.
For example, researchers at Yale University and collaborators from Brazil analyzed Oxitec’s 2015 release of OX513A in Brazil. The scientists confirmed that some offspring of the genetically modified mosquitoes — which were supposed to die and not pass new genes to the wild population — survived to adulthood and mated with their native counterparts. Between 10 and 60 percent of the native mosquitoes contained genes from Oxitec, according to the Yale study, which published in Nature in 2019. The paper’s authors concluded they do not know what impacts these mixed mosquitoes have on disease control or transmission, but added that their findings underscore the importance of monitoring the genetics of the insects.
Oxitec disagreed with the findings and responded on the journal’s website. Oxitec told Gizmodo that Yale’s study includes “numerous false, speculative, and unsubstantiated claims and statements about Oxitec’s mosquito technology.” And when Kofler and three other scientists wrote about Oxitec’s Brazil trial in The Conversation, Oxitec pushed to have the article retracted, says Kofler.
“Here we are, like in the final hour, having these conversations that needed to be happening a year ago,” says Kofler.
For this coming release, some Key Largo locals are willing to act on their anger. Daly, for instance, says that if the mosquitoes are deployed in her neighborhood, she’ll try to put insecticide in any box she finds or send it to an expert to test — even if it means getting in trouble with the federal authorities. “I already have my arresting officer and she said she’s gonna clean her handcuffs for me,” she says. “I don’t care.”
Ideally, Daly says, it won’t have to come to that. She and other locals hope to stop Oxitec before the latest mosquitos are delivered. Daly says she has been busy organizing protests — like one that happened recently in Key Largo — and giving out yard signs to residents who don’t want their property used in the trial. “Locals are pissed off. So I have been busy getting the press to cover the local opposition,” Daly wrote in an email to Undark.
“The first flying insect or animal that can actually use our human blood for a friggin trial for a product to come to market without my consent,” Daly says.
“That’s my blood,” she adds. “That’s my son’s blood. That’s my dog’s blood.”
UPDATE: An earlier version of this story incorrectly suggested that the antibiotic tetracycline is used to treat sewage in Florida. While it can be found in sewage, this is the result of the drug being metabolized by users and flushed into the waste stream. It is not used to treat sewage.
Taylor White is a freelance journalist based in Cape Cod, MA and a graduate of the Science, Health & Environmental Reporting Program at the NYU school of journalism. Her work has appeared in NOVA GBH, Dana-Farber Cancer Institute, the American Association for the Advancement of Science, GenomeWeb, Spectrum, and Science Vs.
It is critical to know what concentration of tetracycline is necessary to sustain the GMO female mosquitoes. I suspect the concentration in the sewage stream would be low enough that they would not survive. But just to be safe, maybe Oxitec should have chosen an antibiotic or other compound that is not readily found in the waste stream or otherwise in the environment. In theory, the mutated gene should disappear from the environment as the population of females declines, so there can no longer be any breeding. However, is there a possibility of, for example, baculoviruses or other arboviruses and mosquito-specific viruses, to pick up the mutated gene from the Oxitec population and transfer it to other mosquito or arthropod species, potentially diminishing their numbers, as well? This occurrence would be a real mess if the gene were transferred to populations of beneficial insects.
What date? You’ve been talking about this forever. I want to be there at the finish line cheering these suckers on FFS.
Tetracycline used to treat bacteria at wastewater treatment plants?! Where on earth did you hear that? Tetracycline may very well be present in the sewage as a result of pass through in the urine of someone taking antibiotics, but it certainly isn’t intentionally added to treat bacteria. Advanced wastewater treatment actually relies on bacteria to remove some of the pollutants from wastewater. Prior to reuse as irrigation quality water, the treated effluent is filtered and chlorinated or exposed to UV light to remove or inactivate bacteria and other pathogens. Considering that many wastewater plants treat millions of gallons per day, using tetracycline for treatment would be monumentally expensive!
——- my wife, a biostatistician and epidemiologist at uc berkeley participated in the first trial of gmo mosquitos here in california . “Some locals say they fear being guinea pigs. ” we locals have a much lower incidence of equine encephalitis and malaria because of that trial. you Luddites should be ashamed of yourselves . . .
The GMO Aedes gad worked wonderfully in Piracicaba, Brazil, where the population helped collecting mosquiroes and the incidence of dengue decreased markedly. For those wondering on the effect on predators, Aedes is a foreign species with no predators. The problem in Brazil was the price for small ciries ti keeo up wuth the costs of the program.
The author should have noted that the 2019 paper has more than just Oxitec criticism. The journal has an “expression of concern” on the paper.
It astonishes me how much people want pesticides instead, and call themselves environmentalists.
Can GMO effect birds that eat them?
more than GMO seeds and corn and plants?
And this is how pandemics are created, made possible. Starve predators so they expand their areas of travel, as it were, to “civilized” areas, bringing us their pathologies.
Mosquitos are in no way the kind of threat that justifies the risk of a pandemic. Nope.
Great to see that our elected officials learned the lesson from Covid-19.
OTOH, I must be wrong. The risk I just described is impossible because the coronavirus was not transmitted in any way by animals to humans but deliberately released by the communist Chinese state, right? Ask any Republican; they’ll tell you I got it right the second time.
GMO mosquitoes. Just like the GMO humans being created with each mRNA Covid quasi vax stab. Not for me. I’ll think of those GMO humans when I pick up a bell pepper. Or avoid the GMO spinach.
Do you understand at all what mRNA is and how the human body responds to its introduction? The material in covid vaccines is biologically incapable of changing your genetics.
We Have fought Oxitec for 10 years! Failed experiments in other Countries have not gone unnoticed. The release area in Brazil became the epicenter of the Zika/Microcephaley outbreak with 400+ babies born with shrunken heads & deformed brains. The Caymans, Malaysia and Panama opted out because Oxitec’s mosquitoes flourished creating a more robust species. The EPA ignored our petition with over 250,000 signatures, countless protests and our concerns for our citizens, our fragile eco system and our valued guests. We are asking that they adhere to The International Code Of Ethics From the Nuremberg Tribunals, “No Human Experimentation Without informed Consent!”
GMO business as usual, spawning environmental risks, community costs and private profits. Regulators still “working hard” to make sense of it all, so as to provide an innovation and investor friendly framework…
It is more like “GMO criticism as usual”. If we consider this proposal on its merits, it has a lot going for it. The arguments are made in the article. This is well illustrated with the GMO food issue, where hundreds of products are now carrying the non-GMO project butterfly icon, appealing to people who have no idea what the objections are, as if all genetically modified organisms are somehow the same. Anybody who thinks it’s important to choose a GMO free olive oil is really not thinking clearly.